Which characteristic is shared by the cleavage sites recognized by type II restriction endonucleases (such as EcoRI)?
Answer and explanation
Correct answer: D. They are palindromic double-stranded DNA sequences that read identically 5' to 3' on both strands
Type II restriction endonucleases recognize specific short (typically 4 to 8 base pairs) palindromic sequences possessing twofold rotational symmetry, meaning the sequence on the top strand read 5' to 3' is identical to the sequence on the complementary bottom strand read 5' to 3' (e.g., 5'-GAATTC-3' for EcoRI).
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