Why does an enzyme usually stop working at a high temperature, such as 80 degrees Celsius?

Answer and explanation

Correct answer: A. The shape of its active site changes

  1. Step 1: The active site has a specific three-dimensional shape that fits the substrate. Step 2. High temperature breaks the bonds that hold the protein in shape, so the active site is lost: the enzyme is denatured. Answer: denaturation of the enzyme. Why not the others: Enzymes are not used up, substrate size does not change with heat, and product inhibition is not the main effect here.

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