Which molecular diagnostic technique uses reverse transcriptase followed by thermal cycles of denaturation, annealing, and elongation to detect viral RNA genomes?
Answer and explanation
Correct answer: C. RT-PCR (Reverse Transcription Polymerase Chain Reaction)
- Step 1: RNA cannot be directly amplified by standard thermostable DNA polymerase. Step 2. Reverse transcriptase first synthesizes complementary cDNA from the RNA template, which is then exponentially amplified via PCR cycles. Answer: RT-PCR (Reverse Transcription Polymerase Chain Reaction). Why not the others: Southern blotting detects DNA, SDS-PAGE separates proteins by molecular weight, and Sanger sequencing requires primers.
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