Which vector system is naturally capable of transferring the Ti (tumor-inducing) plasmid into dicotyledonous plant cells for transgenic plant development?
Answer and explanation
Correct answer: A. Agrobacterium tumefaciens
- Step 1: Agrobacterium tumefaciens is a soil bacterium that naturally causes crown gall disease. Step 2. It transfers a specific segment of its Ti plasmid (the T-DNA) into the nuclear genome of the host plant, making disarmed Ti plasmids widely used vectors in plant genetic engineering. Answer: Agrobacterium tumefaciens. Why not the others: E. coli is used for bacterial cloning; B. thuringiensis produces Bt insecticidal crystal proteins.
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