Starch is mixed with amylase at pH 4, 7 and 10. After 2 minutes only the pH 7 tube no longer turns the iodine solution blue-black. What conclusion can be drawn?

Answer and explanation

Correct answer: A. Amylase works best near pH 7

  1. Step 1: Blue-black iodine shows starch is still present.
  2. Step 2: Only the pH 7 tube had no starch left, so amylase is most active near pH 7.
Answer
Amylase works best near pH 7.
Why not the others: Amylase works best at pH 4: the pH 4 tube still had starch. Amylase is destroyed at pH 7: starch has been digested there. Iodine digests starch: iodine is only an indicator.

Practise more Skills and Applying Knowledge.

Free questions with an explanation for every one, and full timed National 5 (Scotland) mock tests.