Why does an enzyme stop working at a high temperature?

Answer and explanation

Correct answer: A. The active site changes shape, so the substrate no longer fits

  1. Step 1: High temperature breaks bonds in the protein. Step 2. The active site is denatured, so the substrate cannot fit. Answer: The active site changes shape, so the substrate no longer fits. Why not the others: The enzyme is not used up in the reaction.

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