Which PCR step separates the two DNA strands, and at about what temperature?
Answer and explanation
Correct answer: D. Denaturation at about 95 degrees C
- Step 1: Heat breaks hydrogen bonds between the strands. Step 2. That takes about 95 degrees C and is called denaturation. Answer: Denaturation at about 95 degrees C. Why not the others: Annealing binds primers at a lower temperature; extension is where Taq polymerase builds new strands.
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