The Polymerase Chain Reaction (PCR), developed by Kary Mullis, involves three repeated thermal cycles in which sequential order?
Answer and explanation
Correct answer: D. Denaturation Annealing Extension
- Step 1: In PCR, double-stranded template DNA is first heated to to break hydrogen bonds and separate the strands (Denaturation).
- Step 2: The temperature is lowered to to permit synthetic oligonucleotide primers to hybridize to their complementary flanking sequences (Annealing).
- Step 3: The temperature is raised to , the optimal temperature for thermostable Taq DNA polymerase to synthesize complementary strands from free dNTPs (Extension).
Answer
Denaturation Annealing Extension.
Why not the others: Primers cannot anneal before strands are denatured; Extension cannot take place before primers anneal; The other permutations violate the thermal sequence of PCR.
Practise more Biotechnology: Principles and Applications.
Free questions with an explanation for every one, and full timed NEET UG mock tests.
More Biotechnology: Principles and Applications questions
- Restriction endonucleases, often referred to as 'molecular scissors', cleave DNA molecules at specific recognition sites that typically exhi…
- In the standard naming convention for the restriction endonuclease EcoRI, what do the letters 'E', 'co', 'R', and the Roman numeral 'I' re…
- In the popular cloning vector , insertion of a foreign gene of interest at the BamHI restriction site causes insertional in…
- During agarose gel electrophoresis of DNA fragments, in which direction do the fragments migrate and how are they separated according to siz…
- Following agarose gel electrophoresis, DNA bands cannot be seen directly under visible light. What stain and illumination source are require…
- The process of cutting out a resolved DNA band from an agarose gel and extracting the purified DNA from the gel slice is termed: