In genetic engineering, which cloning vector is naturally equipped to replicate in both E. coli and yeast (Saccharomyces cerevisiae) because it contains origins of replication from both species?
Answer and explanation
Correct answer: D. Shuttle vector
- Step 1: Standard plasmids like pBR322 carry a ColE1 origin of replication that functions only in bacteria.
- Step 2: A shuttle vector is a hybrid cloning vector constructed to contain two distinct origins of replication (e.g., and the yeast circle origin) along with selectable markers for both hosts.
- Step 3: This allows the vector to 'shuttle' between bacteria (for easy manipulation and amplification) and yeast (for eukaryotic protein expression).
Answer
Shuttle vector.
Why not the others: Cosmid vector is a plasmid carrying bacteriophage lambda cos sites for packaging large DNA fragments; Phagemid carries an M13 filamentous phage origin for single-stranded DNA production; Lambda vector infects bacteria exclusively.
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