What is the primary carboxylation product formed when RuBisCO fixes carbon dioxide in C3 plants?

Answer and explanation

Correct answer: B. 3-Phosphoglyceric acid (3-PGA)

  1. Step 1: RuBisCO catalyzes the addition of to the 5-carbon acceptor ribulose 1,5-bisphosphate (RuBP).
  2. Step 2: This yields a transient, unstable 6-carbon branched intermediate (2-carboxy-3-keto-D-arabinitol 1,5-bisphosphate).
  3. Step 3: The intermediate immediately undergoes hydrolytic cleavage to form two molecules of 3-phosphoglyceric acid (3-PGA), a 3-carbon compound.
Answer
3-Phosphoglyceric acid (3-PGA)
Why not the others: 'Oxaloacetic acid (OAA)' is the 4-carbon primary carboxylation product in C4 and CAM plants, catalyzed by PEP carboxylase; 'Phosphoenolpyruvate (PEP)' is the primary acceptor in C4 plants, not a product; 'Glyceraldehyde 3-phosphate (G3P)' is formed in the subsequent reduction phase of the Calvin cycle requiring ATP and NADPH.

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