During eukaryotic aerobic cellular respiration, which specific stage generates the majority of ATP molecules via chemiosmosis?

Answer and explanation

Correct answer: B. Oxidative phosphorylation across the inner mitochondrial membrane

  1. Step 1: Evaluate ATP yields across aerobic respiration stages.
  2. Step 2: While glycolysis and the citric acid cycle produce 2 ATP each via substrate-level phosphorylation, the electron transport chain and ATP synthase generate approximately 26–28 ATP per glucose via oxidative phosphorylation.
Answer
Oxidative phosphorylation across the inner mitochondrial membrane.
Why not the others: Glycolysis produces only 2 net ATP; the citric acid cycle yields 2 ATP/GTP; pyruvate oxidation yields NADH but 0 direct ATP.

Practise more Molecules, Cells and Molecular Genetics.

Free questions with an explanation for every one, and full timed EmSAT Achieve (UAE) mock tests.