Which enzyme unwinds and separates the double-stranded DNA helix during replication at the replication fork?
Answer and explanation
Correct answer: B. DNA helicase
- Step 1: DNA helicase breaks hydrogen bonds between complementary base pairs to unwind the double helix. Step 2. Topoisomerase relieves supercoiling strain ahead of the fork, and ligase seals phosphodiester nicks. Answer: DNA helicase. Why not the others: Helicase performs the unwinding separation.
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