During which stage of mitosis do sister chromatids separate at their centromeres and migrate toward opposite poles of the spindle apparatus?
Answer and explanation
Correct answer: C. Anaphase
- Step 1: Trace mitotic phases: Prophase (condensation), Metaphase (equatorial alignment), Anaphase (separation), Telophase (re-formation).
- Step 2: During anaphase, separase cleaves cohesin proteins, allowing spindle kinetochore microtubules to pull sister chromatids to opposite poles.
Answer
Anaphase.
Why not the others: In prophase chromosomes condense; in metaphase chromosomes align along the metaphase plate; in telophase nuclear envelopes reassemble.
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